Biotechnological applications of periplasmic expression in E. coli

Biotechnological applications of periplasmic expression in E. coli

Expression of recombinant proteins outside the cytoplasm was pioneered over 20 years ago in bacterial and yeast systems [1-3], opening the way for its current use in a variety of biotechnological applications [4]. The bacterium Escherichia coli remains the preferred host for the extracytoplasmic expression of recombinant proteins because of its compartmentalized cell envelope, which comprises t...

Periplasmic expression of D1.3 Fab antibody in E. coli

Periplasmic expression of Bacillus thermocatenulatus lipase in Escherichia coli in presence of different signal sequences

Efforts to express lipase in the periplasmic space of Escherichia coli have so far been unsuccessful andmost of the expressed recombinant lipases accumulate in the insoluble cell fraction. To evaluate the role ofnative and heterologous signal peptides in translocation of the lipase across the inner membrane of E. coli,the lipase gene (btl2) was cloned downstream of the native ...

A Novel Vector for Expression/Secretion of Properly Folded Eukaryotic Proteins: a Comparative Study on Cytoplasmic and Periplasmic Expression of Human Epidermal Growth Factor in E. coli

Expression of eukaryotic proteins in E. coli often results in their aggregation. Proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. This is not achieved in cytoplasmic expression in E. coli because of the absence of disulfide bonds formation. A novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

Expression of antibody fragments by periplasmic secretion in Escherichia coli.

Antibody-based drugs are increasingly used in the clinic, and their importance is set to escalate in the coming years as more drugs in this class progress through clinical trials. Although many such drugs utilize whole antibodies, others exploit fragments, e.g., fragment antigen binding (Fab') or single-chain fragment variable (scFv), which retain the antigen-binding specificity without the fra...